Live Cell Imaging
Michelle A. Digman, Ph.D.
University of California, Irvine
Director of the W.M. Keck Nanoimaging Lab
Laboratory for Fluorescence Dynamics (LFD)
Dept.of Developmental and Cell Biology
My research interests focus on 1) quantifying spatial and temporal dynamics of proteins during cell migration and
2) developing novel imaging technologies.
Cell migration is vital for embryonic development, wound healing, and cancer metastasis. We are using the cutting edge biophysical tools to monitor molecular interactions in live cells and how their activity in spatial locations mediates cell migration. Using the raster image correlation spectroscopy (RICS) method and the Number and Molecular Brightness (N&B) analysis we have revealed the dynamic assembly and disassembly of focal adhesions as well as determined the aggregation and stoichimonetry of protein interaction. In particular we are investigating tumor invasion and executing a strategy to monitor signaling for cells migrating in 3D matrices and in tissues.
Developed at the Laboratory for Fluorescence Dynamics (LFD):
Orbital scanning and Line Scanning Fluorescence Correlation Spectroscopy (FCS)
Raster Image Correlation Spectroscopy (RICS)
Pair Correlation Function (pCF)
Number and Brightness Analysis (N&B)
Photon Counting Histogram (PCH)
The Phasor Approach to FLIM
Modulation Tracking/ 3D Single Particle Tracking
Fluorescence Correlation Spectrsocopy (FCS)
Image Correlation Spectroscopy (ICS)
Spatio-Temporal Image Correlation Spectroscopy (STICS)
k-Space Image Correlation Spectroscopy (kICS)
Fluorescence Lifetime Imaging Microscopy (FLIM)
fluorescence intensity distribution analysis (FIDA)
There are many others and if you like them to be listed here, just send me a message.
Cell Migration in 3D
Cell Migration in 2D
Cell Migration in tissues